the comparative effect of fasting with and without caloric restriction in rat on oxidative stress parameters

introduction: fasting, like islamic ramadan fasting, has been associated with health benefits. islamic ramadan fasting, a form of caloric restriction (cr) or alternate day fasting that. studies suggest a comparable effect of adf and caloric restriction. despite the fact that fasting can be considered as a form of dietary restriction, fasters tend to have difficulty to reduce their food intake during non-fasting period by overeating leading to the excessive calorie intake. to compare the effect of fasting with and without caloric restriction in sprague dawley rats. methods: the rats were assigned to one of three groups: adf with 70 % calorie intake (30% cr), adf with 100 % calorie intake (0% cr), and adf with 140 % calorie intake (excessive calorie intake) and al (fed ad libitum). all groups were subjected to 6 hour fasting per day (9 a.m. until 3 p.m.) or 15 days. the plasma sample was taken for mda level assessment. urinary 8-oxodg levels were determined by using elisa. results: adf with 30% calorie restriction (f70) group had the lowest mda level. measurement of 8-oxodg level showed that group f70 had the highest production of 8-oxodg. there was an inverse relationship between mda level and 8-oxodg level meaning the lower mda level, the lower 8-oxodg levels were produced. conclusion: adf fasting with 30% caloric restriction reduce the mda level but increase 8-oxodg levels. this study suggest the beneficial effect of fasting requires decrease in overall caloric intake.